Research Projects

The central research Institute of MEDIOGEN owns a source technology for high-efficiency production of probiotics and stabilization of probiotics and patented SP complex coating technology ensuring the high survival rate at intestines upon administration, and it also operates an independent strains bank by securing excellent strains from various specimens.

Probiotics improving vagina environment

Lactobacillus plantarum MG989, Lactobacillus fermentum MG901
Lactobacillus salivarius MG242, Lactobacillus paracasei MG4272, Lactobacillus rhamnosus MG4288

Vaginitis is one of common disease for a woman of childbearing age. For healthy women, lactobacillus prevents bacterial infection by maintaining the vaginal environment at acidic state, but if lactobacillus flora inside vagina loses its balance, a subject becomes susceptible to vaginitis and vulnerable to reoccurrence even after treatments.
MEDIOGEN not only researches and develops probiotics improving vaginal environment and preventing vaginitis but also continues working hard to find beneficial bacteria improving diverse vaginal environment in order to help women recovering and maintaining femininity.
  • Probiotics separated from vagina of healthy Korean women
  • Inhibitory effect was verified against Gardnerella vaginalis, major pathogen of bacterial vaginitis, and Candida albicans, major pathogen of candidiasis vaginitis.
  • Proliferation suppression of pathogenic organism with generation of antibacterial substance in addition to outstanding superficial adherence rate of vaginal epithelial cells.
  • Prevention for reoccurrence of vaginitis by lowering vaginal pH through generation of lactic acid

Lactobacillus spontaneously moves from anus
to introitus vagina through perineum

(Bastani et al., 2012)

Information on developments
  • Strains with excellent antibacterial activity and survival rate at gastrointestinal tract procured
  • Improvement of vaginal environment and preventive effects for vaginitis verified through the use of animal model for vaginitis
  • Clinical tests on vaginitis

Research result
  • Gardnerella vaginalis inhibitory activity of MG989 and MG901
  • Candida albicans inhibitory activity of MG242
  • Survival rate of MG989, MG901 and MG242 at artificial gastric juice
  • Auto-aggregation
  • Hydrophobicity
  • SEM
Journals & Patents
  • Pack et al., 2016. Characterization and inhibitory activity of Lactobacillus plantarum MG989 and Lactobacillus fermentum MG901 isolated from vaginal microbiota of Korean women against Gardnerella vaginalis and Candida albicans, Korean Soc Biotechnol Bioeng J, 31(1): 40-45
  • Kang et al., 2018. In Vitro probiotic properties of Lactobacillus salivarius MG242 isolated from human vagina, Probiotics Antimicro Pro, 2017, 10(2): 343-349
  • Kang et al., 2018. In vitro probiotic properties of vaginal Lactobacillus fermentum MG901 and Lactobacillus plantarum MG989 against Candida albicans, Eur J Obstet Gynecol Reprod Biol, 228:232-237
  • Kim et al., 2019. Probiotic properties and inhibitory activity of lactic acid bacteria isolated from vaginal microbiota of Korean women against Gardnerella vaginalis and Candida albicans, Korean Soc Biotechnol Bioeng J, 34(2): 91-98
  • Registered patent 10-1750468, compositions containing Lactobacillus fermentum MG901 or Lactobacillus plantarum MG989
  • Registered patent 10-1860513, compositions for prevention or treatment of Candidiasis vaginitis containing Lactobacillus salivarius MG242 separated from vagina
  • Patent application 10-2019-0052198, Probiotics for treatment or improvement of vaginitis with antimicrobial effects on Gardnella vaginalis and Candida albicans
  • Patent application 10-2019-0052269, Probiotics MG4288 for treatment or improvement of vaginitis with antimicrobial effects on Gardnella vaginalis and Candida albicans
Functional Probiotics for sugar control

Lactobacillus plantarum MG4229, Lactobacillus plantarum MG4296, Lactobacillus plantarum MG5025 Lactobacillus paracasei MG5012

Diabetes is a disease arising when blood sugar rises due to inability to properly decompose sugar in the blood and a major cause for chronic diseases or complications. The prevalence of adult diabetes for 30 years or older is 12.4% (approx. 3.33 million patients) as of 2017, and the prevalence of fasting blood sugar disorder with high probability to transmigrate diabetes is 26% (approx. 6.97 million patients), which leads diabetes to be classified as a disease with relatively high prevalence. Currently, development of remedy for diabetes with less side effects using lactobacillus is being aggressively pursued.
  • Inhibitory efficacy for α-glycosidase and α-amylase enzyme activity verified
  • Sugar consumption at 3T3-L1 cell verified
Information on Developments
  • Screening of lactobacillus strain featured with sugar lowering function by using carbohydrate digestive enzyme (α-glycosidase)
  • Final selection of outstanding lactobacillus strain through verification of productivity and probiotics tests
  • Measurement of anti-oxidation activity
  • Evaluation of validity of animal glucose tolerance
  • Verification of sugar control function through animal tests
Research result
  • Inhibitory efficacy for enzyme activity verified of α-glycosidase and α-amylase
  • Anti-oxidation effect verified
Lactobacillus inhibiting fat differentiation

Lactobacillus fermentum MG4231, Lactobacillus fermentum MG4244

Obesity may be induced by differentiation of fat cell and excessive supply of energy. Because such obesity is prone to cause various metabolic syndromes, aggressive efforts for weight loss are required. Lately, as efficacy of probiotics to reduce body fat has been discovered, the importance of probiotics is being highly valued as raw materials capable of replacing various chemosynthetic fat differentiation inhibitory materials currently available in the market. MEDIOGEN endeavors to develop products helping reduction of body fat by developing lactobacillus inhibiting differentiation activity of fat cell and effectively suppressing accumulation of neutral fat in human body.
  • Suppression of differentiation of 3T3-L1 cell, fat precursor cell, to fat cell
  • Effects of high fat diet to reduce weight and subcutaneous fat
Information on developments
  • Animal model test on high fat diet
  • Screening of candidates for suppression of fat cell differentiation
  • Individual certification of raw materials
Research results
  • Verification of inhibition of fat cell differentiation by 3T3-L1
  • Verification of liver biopsy
  • Verification of anti-obesity effects at liver cell through animal tests
Journals & Patents
  • Registered patent 10-1981333, Human-derived Lactobacillus fermentum MG4231 with anti-obesity activation or Lactobacillus fermentum MG4244 and composition containing the above
  • Kang et al., 2018. In vitro probiotic evaluation of potential antiobesity lactic acid bacteria isolated from human vagina and shellfish, Korean Soc Biotechnol Bioeng J, 33(3): 161-167
Oral functional probiotics
Major causative bacteria for oral disease include Streptococcus mutans, Streptococcus sobrinus, Porphyromonas gingivalis, Treponema denticola. Especially, S. mutans and S. sobrinus are known to cause periodontal disease forming dental plaque using glucosyltranferase. With surging interests in oral cleanliness and increasing demands for related products in recent years, a variety of products inhibiting harmful oral bacteria are being introduced in the market. But because these mouth cleaning agents also sanitize beneficial oral bacteria and hinder bactericidal effects at the core regions where causative bacterial for cavity actively proliferate, the needs to develop products supplementing the above are being stressed.
  • Inhibition of proliferation of S. mutans and P. gingivalis, major causative bacterial, for oral diseases
  • Decomposition or suppression of generation of volatile sulfur compound, the cause of bad breath
  • Inhibition of formation of biofilm at tooth surface
Information on developments
  • Verification of resistant capacity against lysozyme within oral environment
  • Verification of antibacterial activity from S. mutans and P. gingivalis, causative bacterial for oral diseases
  • Verification of inhibitory activity against formation of biofilm through Autoaggregation
  • Probiotics functionality (Autoaggregation, GI tract, Adhesion, Hemolysis, antibiotics tolerance, etc.)
Research results
  • Verification of inhibition ratio for proliferation of Streptococcus mutans, causative bacterial for oral diseases
Journals & Patents
  • Registered patent 10-2001074, Lactobacillus composition equipped with cavity inhibitory activity
  • Kang et al., 2018. Anticariogenic activity and probiotic properties of lactic acid bacteria isolated from human and fermented foods, 33(4): 1-6
Liver functional lactobacillus
Drinking must be the major cause threatening human liver, today. While acetaldehyde remained in a body after liver decomposes alcohol incurs hangover, the demands for alcohol detoxification beverage continue increasing to address this matter. Accordingly, MEDIOGEN aims to develop functional lactobacillus products specialized to improve liver function by carefully selecting lactobacillus aggressively decomposing alcohol and acetaldehyde.
  • Strong tolerance against ethanol
  • Improvement of liver function and hangover cures by using lactobacillus strains with superb resolution of ethanol and acetaldehyde
Information on developments
  • Verification of alcohol tolerance and selection of strains displaying acetaldehyde resolution
  • Verification through animal tests
  • Verification through probiotics experiments
Research result
  • Verification of acetaldehyde generation following ethanol decomposition
  • Verification of results from ethanol tolerance
Journals & Patents
  • Kim et al., 2003. Effect of Lactobacillus fermentum MG590 on alcohol metabolism and liver function in rats. J Microbiol Biotechnol, Korean Soc Biotechnol Bioeng J, 13(6): 919-925